pseudoacacia growth is resistant to increased drought frequency because it employs two strategies – drought tolerance or drought avoidance, followed by compensation. Conversely, under high-frequency droughts, plants fixed C and N at low rates during dry phases and at high rates during wet phases. Under low-frequency droughts, plants fixed carbon (C) and nitrogen (N) at similar rates during wet and dry phases. However, seedlings responded differently to wet and dry phases depending on drought frequency. We found no effect of drought frequency on final biomass or mean SNF rate.We grew Robinia pseudoacacia seedlings under the same mean soil moisture, but with different drought frequency caused by wet–dry cycles of varying periodicity.Here, we assessed the effect of drought frequency on the ecophysiology and SNF rate of a common N2-fixing tree in eastern US forests. Climate change is increasing drought frequency, which may affect symbiotic N2 fixation (SNF), a process that facilitates ecosystem recovery from disturbance.